Physics Colloquium: Hybrid Solution and Solid-State NMR Approach to Determine the High-Resolution Structure and Topology

Event Details

  • Speaker(s): Dr. Gianluigi Veglia
  • Date:
  • Time: 9:15 a.m.
  • Location: SCIE 1511

Speaker: Dr. Gianluigi Veglia
Department of Biochemistry, Molecular Biology & Biophysics
University of Minnesota

Abstract

A novel hybrid approach for the structure calculations of membrane proteins using restraints derived from solution and solid-state NMR will be presented. This hybrid method combines restraints from solution NMR experiments carried out in detergent micelles (nuclear Overhauser effects, paramagnetic relaxation enhancements, residual dipolar couplings, and 3J couplings) with anisotropic parameters derived from solid-state NMR in oriented DOPC/DOPE lipid bilayers (1H-15N dipolar couplings and 15N chemical shift anisotropy) for the imultaneous determination of high-resolution structure and topology of membrane proteins. All of the structural restraints are incorporated into a single structural refinement protocol using XPLOR-NIH software. We show the application of this method to phospholamban (PLN), a pentameric integral membrane protein involved in muscle contraction and relaxation. We propose this approach as a general method for structure determination of membrane proteins at high-resolution.

For more information, contact:

Department of Physics
University of Guelph
Guelph, Ontario
N1G 2W1
Phone: (519) 824-4120 Ext. 52261
Fax: (519) 836-9967
http://www.physics.uguelph.ca