Nanoscience: Fluorescence Spectroscopy

Cary Eclipse Spectrophotometer

Fluorescence spectroscopy measures the emission of a photon when an excited electron in a chromophoric species returns to the ground state. The energy of the emitted photon is lower (i.e. red shifted) than the photon used to promote the electron to higher virtual state. The overall fluorescence intensity of a given species in a given environment depends on its quantum yield and is proportional concentration at low analyte concentrations.

Cary Eclipse Fluorescence Spectrophotometer

Features

  • Wavelength Range: 190 nm to 1100 nm
  • Xenon pulse lamp minimizes photobleaching and noise
  • Highly sensitive PMTs with low, medium and high gains
  • Spectral Resolution of 1.5 nm
  • Variable Slit Widths
  • Multicell Holder for measuring 4 cuvettes per run
  • Temperature Ranges from -10oC to 100oC
  • Can operate in Analytical and Kinetic Modes

Applications

  • Pharmaceuticals
  • Dye Formulations and Stains
  • Organic Compound Identification and Quantification<
  • Ligand Binding
  • Liquid Crystals and Aluminates
  • Nanoparticles and Quantum Dots
  • Semiconductors
  • Enzyme Kinetics
  • Environmental and Toxicological Studies

Examples

Graph Description
Fluorescence peak shifts for CdSe quantum dots of varying size Fluorescence peak shifts for CdSe quantum dots of varying size
Quenching of pyrene fluorescence with increasing concentrations of dodecylpyridinium chloride Quenching of pyrene fluorescence with increasing concentrations of dodecylpyridinium chloride
Leakage rates for Sephadex G-10 beads as function of the adsorbed phospholipid coating Leakage rates for Sephadex G-10 beads as function of the adsorbed phospholipid coating