Nanoscience: Fluorescence Spectroscopy
Cary Eclipse Spectrophotometer
Fluorescence spectroscopy measures the emission of a photon when an excited electron in a chromophoric species returns to the ground state. The energy of the emitted photon is lower (i.e. red shifted) than the photon used to promote the electron to higher virtual state. The overall fluorescence intensity of a given species in a given environment depends on its quantum yield and is proportional concentration at low analyte concentrations.
Features
- Wavelength Range: 190 nm to 1100 nm
- Xenon pulse lamp minimizes photobleaching and noise
- Highly sensitive PMTs with low, medium and high gains
- Spectral Resolution of 1.5 nm
- Variable Slit Widths
- Multicell Holder for measuring 4 cuvettes per run
- Temperature Ranges from -10oC to 100oC
- Can operate in Analytical and Kinetic Modes
Applications
- Pharmaceuticals
- Dye Formulations and Stains
- Organic Compound Identification and Quantification<
- Ligand Binding
- Liquid Crystals and Aluminates
- Nanoparticles and Quantum Dots
- Semiconductors
- Enzyme Kinetics
- Environmental and Toxicological Studies
Examples
Graph | Description |
---|---|
Fluorescence peak shifts for CdSe quantum dots of varying size | |
Quenching of pyrene fluorescence with increasing concentrations of dodecylpyridinium chloride | |
Leakage rates for Sephadex G-10 beads as function of the adsorbed phospholipid coating |