Nanoscience: UV-visible Absorbance Spectroscopy

Cary 300Bio UV-Visible Spectrophotometer

UV-visible spectroscopy is measures the degree of transmittance or reflection by a chromophoric species in the presence of UV and visible radiation. The absorbance (loss in incident energy) is a measurement of the electronic transistion of a ground state electron to an excited state. The absorbance can be used to quantify the concentration of an analyte in a given sample according to Beer's Law (A ∝ c)

Cary 300Bio UV-Visible Spectrophotometer

Features

  • Wavelength Range: 190 nm to 900 nm
  • High Sensitivity: above 5.0 absorbance units
  • Spectral Resolution of 0.2 nm
  • Dual Beam Operation
  • Variable Slit Widths
  • Can operate in Analytical and Kinetic Modes
  • Can perform Reflectivity Measurements of Solid Substrates

Applications

  • Pharmaceuticals
  • Dye Formulations & Organic Compounds
  • Ligand Binding
  • Nanoparticles & Quantum Dots
  • Semiconductors
  • Plasmonics
  • Plasmonics & Enzyme Kinetics
  • Environmental and Toxicological Studies

Examples

Image Description
Graph indicating UV absorbance spectra of SPF 30 (red) and SPF 50 (blue) sunscreens UV absorbance spectra of SPF 30 (red) and SPF 50 (blue) sunscreens
Graph indicating Plasmonic absorbance peaks for gold and silver nanoparticles Plasmonic absorbance peaks for gold and silver nanoparticles
Graph indicating UV Abs.. spectra of ZnO quantum dots as a function of growth time UV Abs. spectra of ZnO quantum dots as a function of growth time