Nanoscience: UV-visible Absorbance Spectroscopy
Cary 300Bio UV-Visible Spectrophotometer
UV-visible spectroscopy is measures the degree of transmittance or reflection by a chromophoric species in the presence of UV and visible radiation. The absorbance (loss in incident energy) is a measurement of the electronic transistion of a ground state electron to an excited state. The absorbance can be used to quantify the concentration of an analyte in a given sample according to Beer's Law (A ∝ c)
Features
- Wavelength Range: 190 nm to 900 nm
- High Sensitivity: above 5.0 absorbance units
- Spectral Resolution of 0.2 nm
- Dual Beam Operation
- Variable Slit Widths
- Can operate in Analytical and Kinetic Modes
- Can perform Reflectivity Measurements of Solid Substrates
Applications
- Pharmaceuticals
- Dye Formulations & Organic Compounds
- Ligand Binding
- Nanoparticles & Quantum Dots
- Semiconductors
- Plasmonics
- Plasmonics & Enzyme Kinetics
- Environmental and Toxicological Studies
Examples
Image | Description |
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UV absorbance spectra of SPF 30 (red) and SPF 50 (blue) sunscreens | |
Plasmonic absorbance peaks for gold and silver nanoparticles | |
UV Abs. spectra of ZnO quantum dots as a function of growth time |